| Popis: |
Na crioconservação de sêmen canino, técnicas de congelamento precisam ser otimizadas com a finalidade de maximizar a viabilidade do sêmen após a descongelação. O ejaculado de um animal foi congelado em palhetas de 0,5ml utilizando como diluidores o Tris-citrato-frutose, Tris-citrato-glucose, com 6% de glicerol no volume final, e o diluente Merk. As amostras foram avaliadas imediatamente após descongelação quanto à motilidade e integridade de acrossomas. As mais altas taxas de motilidade e integridade acrossomal foram obtidas com o diluente Tris-citratoglucose, havendo diferenças significativas quando comparadas com os demais diluentes, demonstrando a variação na viabilidade do sêmen de um mesmo animal frente a diferentes diluidores. Preliminary study on some properties of canine frozen semen Abstract Current techniques for freezing canine semen ought to be improved in order to attain maximum viability from ejaculates. In the present study, three aliquots of an ejaculate from one dog has been diluted in three different extenders: TRIS-citrate-fructose, TRIS-citrate-glucose and Merck extender. Glycerol in the final concentration of 6% has been added to TRIS-citrate-fructose and TRIS-citrate-glucose extenders after the semen dilution. After the dilution, all three preparations were cooled first at 5ºC and then frozen in liquid nitrogen at 196ºC. Spermatozoa motility and acrosomal integrity were assessed immediately after thawing. The values for the rate of motility and the proportion of spermatozoa displaying intact acrosome found in the TRIS-citrate-glucose preparation were significantly superior when compared with the values found for TRIS-citrate-fructose and the Merk extender. |
