Qualitative and Quantitative Detection of Protein and Genetic Traits in Genetically Modified Food

Autor:	A. Papathoma, Panayotis Markoulatos, B. Betzios, N. Siafakas, Maurice L. J. Moncany, E. Nerantzis, Vassilis Dourtoglou
Rok vydání:	2004
Předmět:	business.industry General Chemical Engineering Genetic traits media_common.cataloged_instance Genetically modified crops European union Biology business Genetically modified food Food Science Biotechnology media_common Genetically modified organism
Zdroj:	Food Reviews International. 20:275-296
ISSN:	1525-6103 8755-9129
DOI:	10.1081/fri-200029418
Popis:	Due to the market introduction of genetically modified organisms (GMOs) in crops, foods, and ingredients, legislation worldwide came face to face with the question of the use and labeling requirements on GMO crops and their derivatives. In this review, protein- and DNA-based methods, such as enzyme-linked immunosorbent assay, western blots, and qualitative and quantitative polymerase chain reaction PCR (Q-PCR) are reviewed. Qualitative detection methods for genetically modified (GM) sequences in foods have evolved rapidly during the past years. The sensitivity of these systems is extremely high, even for processed foodstuffs. However, the availability of quantitative detection methods for GMO analysis is an important prerequisite for the introduction of threshold limits for GMOs in food. The recently introduced labeling threshold for GMOs in food ingredients by the European Union has forced official food control laboratories to apply quantitative PCR methods. Taking the precision of quantitative ...
Databáze:	OpenAIRE
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