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Previous attempts have been made to integrate gene amplification (PCR) with electrophoretic separation of PCR products on a chip fabricated in PDMS. PDMS is a widely used elastic polymer for rapid prototyping using soft lithography. In its pre-cure stage, PDMS consists of multiple components and after curing, the polymerization reactions are usually not competed. Consequently, there are low molecule weight compounds present in the PDMS replica. Environmentally dependent surface changes as well as surface contamination for many polymers are well known. All these lead to changes in surface properties. In this study, the surface properties of PDMS were studied. We have detected the presence of volatile oligosiloxanes in the interface between a PDMS replica and a glass substrate. Also, their behavior at room and elevated temperatures, in particular the adhesion strength between the PDMS-glass interface, was analyzed.© (2002) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only. |
