Viral Hepatitis B: Seroprevalence and Genetic Diversity in Blood Donors North of Congo

Autor:	Amelia Dzia Lepfoundzou, Mouakosso Marlyse Ngalessami, Adoua Cs, Blaise Irénée Atipo Ibara, Jile Florent Mimiesse Monamou, Jean-Rosaire Ibara, Ahoui Apendi Clausina Philestine, Arnaud Mongo-Onkouo, Rody Stéphane Ngami, Ngala Akoa Itoua-Ngaporo, Dinghat Ornela Marlyne Yvonne, Deby Gassaye
Rok vydání:	2019
Předmět:	Genetic diversity HBsAg medicine.medical_specialty Blood transfusion business.industry medicine.medical_treatment medicine.disease Virology Serology Genotype Epidemiology Medicine Seroprevalence business Viral hepatitis
Zdroj:	Open Journal of Gastroenterology. :239-245
ISSN:	2163-9469 2163-9450
DOI:	10.4236/ojgas.2019.912027
Popis:	Introduction: Viral hepatitis B is the most formidable and confusing of all viral hepatitis, given its cirrhogenic and carcinogenic potential. The objective of the study is to characterize the molecular profile of hepatitis virus B in northern Congo. Materials and Methods: This was a descriptive and cross-sectional study that was carried out between January and September 2014, that is 9 months, in the establishments of the National Center for Blood Transfusion (CNTS) of the 4 departments of the north of the Congo. Epidemiological, serological and molecular variables (HBsAg, HBV DNA, genotypes and subtypes) were studied. The HBs antigen was searched by rapid test and confirmed by ELISA. For all positive donors, conventional and specific gene extraction and amplification techniques were performed for the identification of genotypes and subtypes from the serum. Results: A total of 892 donors were included. The average age was 35.36 ± 12.36 years with extremes ranging from 18 to 65 years old. The sex ratio (M/F) was 3.3. The prevalence of HBV was 8.6%. The viral DNA of HBV was amplified in 83.1%. Four genotypes were found: E (40.6%), A (3.1%), B (3.1%) and C (1.6%). Two cases of co-infection were identified: E/A (15.6%) and B/C (1.6%). Subgenotypes A1, B2 and C1 were highlighted. Conclusion: The prevalence of HBV is high, HBV infection remains a major public health problem for blood donors in Congo. Genotypes E, A, B, C as well as subtypes A1, B2, C1 have been identified in northern Congo.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::cf40fb954869ad314a6c23f6efb8885a https://doi.org/10.4236/ojgas.2019.912027 Zobrazit plný text záznamu