Timothy Grass (Phleum pratense L.) Pollen as Allergen Carriers and Initiators of an Allergic Response
| Autor: | W Sliwa-Tomczok, J. Ring, Wolf-Meinhard Becker, J Tomczok, Heidrun Behrendt, A. Kasche, C. Ebner von Eschenbach |
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| Rok vydání: | 1999 |
| Předmět: | |
| Zdroj: | International Archives of Allergy and Immunology. 118:414-418 |
| ISSN: | 1423-0097 1018-2438 |
| DOI: | 10.1159/000024151 |
| Popis: | Contrary to indoor allergen exposure (e.g. house dust mite), there is no reliable quantitative association between pollen exposure and symptoms of allergic diseases. Therefore we studied localization and release of major allergens from timothy grass (Phleum pratense L.) pollen using different methods and pollen grain sources. Localization of major allergens Phl p 5 and Phl p 1 was visualized by field emission scanning electron microscopy after anhydrous fixation and immunogold silver staining in a three–dimensional reconstruction; Phl p 5 was found in the cytoplasm and on the exine, Phl p 1 in the intine. No allergens were found inside the starch granules. Allergen liberation from pollen grains was studied in vitro under physiological conditions (30 min, 37°C) at pH 6.0, 7.4 and 9.0. Besides total protein measurements in the supernatant, major allergens were determined by immunoblot, Phl p 5 was quantitated by ELISA. There were striking differences in total protein and major allergen release between freshly collected and commercially available grass pollen grains as well as among freshly collected pollen between rural meadows and areas near high–traffic roads. There was a significantly different release of total protein being lowest in supernatants from commercially available pollen grains (rural/traffic vs. commercial, ptraffic>commercial, p |
| Databáze: | OpenAIRE |
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