Six-Transmembrane Epithelial Antigen of the Prostate-1 (STEAP-1)-Targeted Ultrasound Imaging Microbubble Improves Detection of Prostate Cancer In Vivo
| Autor: | Jing Hu, Chen-Yang Zhao, Ying Liu, Xiang-Ming Zhu, Feng Jiang, Yun Yuan |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
030219 obstetrics & reproductive medicine
Radiological and Ultrasound Technology biology business.industry medicine.drug_class Ultrasound Monoclonal antibody biology.organism_classification medicine.disease 030218 nuclear medicine & medical imaging 03 medical and health sciences Prostate cancer 0302 clinical medicine medicine.anatomical_structure Nude mouse Antigen Prostate In vivo Microbubbles Cancer research Medicine Radiology Nuclear Medicine and imaging business |
| Zdroj: | Journal of Ultrasound in Medicine. 38:299-305 |
| ISSN: | 0278-4297 |
| DOI: | 10.1002/jum.14689 |
| Popis: | PURPOSE To investigate the feasibility of the 6-transmembrane epithelial antigen of the prostate-1 (STEAP-1)-targeted microbubbles for enhancing ultrasound imaging of prostate tumors in the nude mouse xenograft models. METHODS Contrast agents were established by conjugating biotinylated STEAP-1 monoclonal antibodies with streptavidin coated SonoVue microbubbles. Then, ordinary SonoVue (Bracco, Milan, Italy) microbubble and STEAP-1-targeted SonoVue microbubble were used, respectively, for contrast-enhanced sonography to detect prostate tumors in the nude mouse xenograft models. The characteristics, including peak intensity, time to peak, area under the curve, and mean transit time, were measured. RESULTS The biological characteristics of STEAP-1-targeted SonoVue microbubbles were stable. STEAP-1-targeted SonoVue microbubbles can successfully conjugate to prostate cancer cell lines in vitro. Enhancement of ultrasound signal intensity was determined after injection of STEAP-1-targeted SonoVue microbubble, compared with ordinary SonoVue microbubble. Higher intensities of ultrasound signals in xenograft tumor of prostate cancer were associated with increased levels of STEAP-1 expression. CONCLUSION Our results suggest that SonoVue microbubble carrying STEAP-1 monoclonal antibody could improve the ultrasound visualization of prostate cancer and identify the tumor more effectively in vivo. A prospective study is required to validate our finding in patients with prostate cancer. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text