Effect of lipids and detergents on the production and solubilization of extracellular lipase inYarrowia lipolytica

Autor:	l. Doležalová, Č. Novotnyý
Rok vydání:	1993
Předmět:	chemistry.chemical_classification Chromatography biology Triacylglycerol lipase General Medicine Microbiology Oleic acid chemistry.chemical_compound Digitonin Enzyme chemistry Biosynthesis Ionic strength biology.protein Urea Lipase
Zdroj:	Folia Microbiologica. 38:49-54
ISSN:	1874-9356 0015-5632
DOI:	10.1007/bf02814549
Popis:	Triglycerides, oleic acid but not fatty acid-containing, nonionic detergents (Spans, Tweens) were able to stimulate the synthesis of cell-bound and soluble lipase ofYarrowia lipolytica grown in a complex medium containing citrate and urea. The optimal concentration of olive oil for induction was 0.5% (W/V). The combined effect of a high ionic strength (0.75 mol/L KCl) and of digitonin (2 mmol/L) at pH 7.6 resulted in solubilization of 80% of the cell-bound lipase and a significant activation of the enzyme. Comparison of twoY. lipolytica strains showed the effects of Mg2+ and Fe3+ concentrations in the medium on the synthesis of the enzyme to be strongly strain-dependent.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::cf1e5f573df006cec75d41ba57adb643 https://doi.org/10.1007/bf02814549 Zobrazit plný text záznamu Full text from SpringerLink