Infection of Nicotiana benthamiana with Uncut Cloned Tandem Dimers of Tomato Golden Mosaic Virus DNA

Autor:	Robert H.A. Coutts, K. W. Buck, R. J. Hayes, C. L. Brough, V. E. Prince
Rok vydání:	1988
Předmět:	biology Nicotiana benthamiana Molecular cloning biology.organism_classification Virology Molecular biology Virus law.invention chemistry.chemical_compound Plasmid chemistry Capsid law Recombinant DNA DNA supercoil DNA
Zdroj:	Journal of General Virology. 69:209-218
ISSN:	1465-2099 0022-1317
DOI:	10.1099/0022-1317-69-1-209
Popis:	Summary Recombinant plasmids containing dimeric inserts of tomato golden mosaic virus (TGMV) DNA component A (pA2), DNA component B (pB2) and both DNA components (pA2B2) were constructed. When inoculated onto Nicotiana benthamiana plants, mixtures of uncut pA2 and pB2, or uncut pA2B2 alone, induced symptoms typical of TGMV infection. Infections induced by the uncut dimeric clones were very similar to those induced by the excised monomeric clones, as judged by symptom development, production of capsid protein, immunological reaction with antiserum to TGMV, production of circular double-stranded DNA and single-stranded DNA of genome size, and restriction analysis of supercoiled DNA. Infection with the dimeric DNA clones gave rise to a subgenomic DNA, derived from DNA A, which was not observed in infections with monomeric clones. The infectivity of excised monomeric DNA was shown to be dependent on the cloning site, but the uncut dimeric clones were as infectious as the most infectious monomeric clones.
Databáze:	OpenAIRE
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