A miRNA-based classification of renal cell carcinoma subtypes by PCR andin situhybridization
| Autor: | Kalra Krishan, Ashley Di Meo, Qiang Ding, George M. Yousef, Eleftherios P. Diamandis, Samantha Wala, Haiyan Zhai, Heba W.Z. Khella, Adriana Krizova, Manal Y. Gabril, Antonio Finelli, Rola Saleeb, A. Evans, Fadi Brimo, Maria D. Pasic |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty business.industry In situ hybridization Chromophobe cell Biology urologic and male genital diseases medicine.disease 3. Good health 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Text mining Oncology Renal cell carcinoma 030220 oncology & carcinogenesis microRNA medicine Oncocytoma business Renal oncocytoma Clear cell |
| Zdroj: | Oncotarget. 9:2092-2104 |
| ISSN: | 1949-2553 |
| Popis: | Renal cell carcinoma (RCC) constitutes an array of morphologically and genetically distinct tumors the most prevalent of which are clear cell, papillary, and chromophobe RCC. Accurate distinction between the typically benign-behaving renal oncocytoma and RCC subtypes is a frequent challenge for pathologists. This is critical for clinical decision making. Subtypes also have different survival outcomes and responses to therapy. We extracted RNA from ninety formalin-fixed paraffin-embedded (FFPE) tissues (27 clear cell, 29 papillary, 19 chromophobe, 4 unclassified RCC and 11 oncocytomas). We quantified the expression of six miRNAs (miR-221, miR-222, miR-126, miR-182, miR-200b and miR-200c) by qRT-PCR, and by in situ hybridization in an independent set of tumors. We developed a two-step classifier. In the first step, it uses expression of either miR-221 or miR-222 to distinguish the clear cell and papillary subtypes from chromophobe RCC and oncocytoma (miR-221 AUC: 0.96, 95% CI: 0.9132-1.014, p < 0.0001 and miR-222 AUC: 0.91, 95% CI: 0.8478-0.9772, p < 0.0001). In the second step, it uses miR-126 to discriminate clear cell from papillary RCC (AUC: 1, p < 0.0001) and miR-200b to discriminate chromophobe RCC from oncocytoma (AUC: 0.95, 95% CI: 0.8933-1.021, p < 0.0001). In situ hybridization showed a nuclear staining pattern. miR-126, miR-222 and miR-200b were significantly differentially expressed between the subtypes by in situ hybridization. miRNA expression could distinguish RCC subtypes and oncocytoma. miRNA expression assessed by either PCR or in situ hybridization can be a clinically useful diagnostic tool to complement morphologic renal tumor classification, improving diagnosis and patient management. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|