Time course of a dedifferentiation process during isolation and culture of protoplasts: Comparison between protoplasts from young and mature regions of the mung bean hypocotyl

Autor: Monique Pierron, Renée Goldberg, Jean-Paul Dubacq, Véronique Thor, Roger Prat
Rok vydání: 1986
Předmět:
Zdroj: Plant Science. 46:63-68
ISSN: 0168-9452
DOI: 10.1016/0168-9452(86)90132-9
Popis: Protoplasts were isolated from successive sections along the mung bean hypocotyl. High yields were obtained with mature tissues. The fatty acid composition of the protoplasts was similar to that found in the original cells which indicates that no major structural alteration of membranes occurs during protoplast isolation. In contrast, all the cells regenerated from the protoplasts exhibited a modified lipid composition and isoperoxidase profiles when compared to hypocotyl cells.
Databáze: OpenAIRE