Enzymatic Lysis of Living Microbial Cells: A Universal Approach to Calculating the Rate of Cell Lysis in Turbidimetric Measurements

Autor:	Vladimir I. Tishkov, D. L. Atroshenko, D. A. Matolygina, N. G. Belogurova, E. D. Ovchinnikova, N.L. Eremeev, S. S. Savin, P. A. Levashov, Andrey V. Levashov, N. S. Dushutina, S. A. Smirnov
Rok vydání:	2018
Předmět:	0301 basic medicine chemistry.chemical_classification 03 medical and health sciences 030104 developmental biology Lysis medicine.anatomical_structure Chromatography Enzyme Chemistry Cell medicine General Chemistry
Zdroj:	Moscow University Chemistry Bulletin. 73:47-52
ISSN:	1935-0260 0027-1314
Popis:	The data of the turbidimetric measurement for the enzymatic lysis of various living bacterial cells are analyzed. A method for the correct recalculation of the turbidimetric data (–ΔA/Δt) into absolute values of the change in the concentration of living cells (–ΔCFU/Δt) is proposed. The dimensionless efficiency of cell lysis–(1/CFU0) · ΔCFU/Δt for various bacterial cells is calculated to correctly compare the efficiency of the action of different bacteriolytic factors on various bacterial cells.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ceee06742c44fdf06aaf38a82776bf3b https://doi.org/10.3103/s0027131418020104 Zobrazit plný text záznamu Full text from SpringerLink