Effect of l-Phenylalanine on Rooting of Shoots from Axillary Buds of Adult Larix Leptolepis

Autor: Shinjiro Ogita, Masami Fushitani, Takafumi Kubo
Rok vydání: 1997
Předmět:
Zdroj: Journal of Forest Research. 2:217-220
ISSN: 1610-7403
1341-6979
DOI: 10.1007/bf02348318
Popis: Rooting of shoots derived from axillary buds was examined to establish an efficient shoot culture system of clonal micropropagation in adult tree ofLarix leptolepis Gord. (Japanese larch). Nine out of ten shoots induced calli (90%) on their shoot bases, and the two of them formed root primordia with a red pigment (20%) on the calli surface within 5 weeks after culturing on modified Murashige and Skoog (MS) medium supplemented with 1.5 μM of indolebutyric acid (IBA) and 1.5 μM of naphthaleneacetic acid (NAA). However, the primordia did not elongate actively. The addition of 10 mMl-phenylalanine in the MS medium with the auxins resulted in the formation of roots at high frequency, about 80%, and they elongated actively. Although callus was formed in all the shoots cultured on the medium withl-phenylalanine, it appeared that the callus development was less as compared to the medium withoutl-phenylalanine. Consequently, the rooting might be associated with the suppression of the induced callus.
Databáze: OpenAIRE
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