Cellulose as a template to fabricate a cellulase-immobilized composite with high bioactivity and reusability
| Autor: | Lin Tan, Jianhui Qiu, Zhaojun Tan, Huixia Feng |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
biology
010405 organic chemistry Composite number technology industry and agriculture 02 engineering and technology General Chemistry Cellulase 021001 nanoscience & nanotechnology 01 natural sciences Catalysis 0104 chemical sciences Chitosan chemistry.chemical_compound Chemical engineering chemistry Immobilized cellulase Materials Chemistry biology.protein High activity Cellulose 0210 nano-technology Fe3o4 nanoparticles Reusability |
| Zdroj: | New Journal of Chemistry. 42:1665-1672 |
| ISSN: | 1369-9261 1144-0546 |
| DOI: | 10.1039/c7nj03271d |
| Popis: | Herein, a new strategy was developed to fabricate an oriented cellulase/chitosan/Fe3O4 composite, which possesses extremely high activity, reusability, and stability. Compared to the conventional methods, herein, cellulase was first mixed with cellulose; this allowed the active-centers of cellulase to combine with cellulose tightly. Then, the cellulase/cellulose mixture was immediately embedded into chitosan (CS) layers, which were coated with Fe3O4 nanoparticles. After hydrolyzing the cellulose, a flexible hollow structure was obtained. The activity of immobilized cellulase was still maintained over 50% even after 15 uses; this indicated that the composite we fabricated herein possessed much higher activity and reusability as compared to those reported in other studies. The promising stability and activity of the immobilized cellulase is very important for practical applications. |
| Databáze: | OpenAIRE |
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