Exploiting Expressed Sequence Tag Databases for the Development and Characterization of Gene-Derived Simple Sequence Repeat Markers in the Opium Poppy (Papaver somniferum L.) for Forensic Applications

Autor:	Moon Sik Yang, Eun-Jung Lee, Gang Nam Jin, Yang-Han Lee, Myun Soo Han, Kyung Lyong Lee
Rok vydání:	2011
Předmět:	Genetics Expressed sequence tag biology Database food and beverages Locus (genetics) Opium Poppy biology.organism_classification computer.software_genre Eschscholzia Pathology and Forensic Medicine Loss of heterozygosity Papaver Microsatellite Allele computer
Zdroj:	Journal of Forensic Sciences. 56:1131-1135
ISSN:	0022-1198
DOI:	10.1111/j.1556-4029.2011.01810.x
Popis:	Simple sequence repeat (SSR) markers in the opium poppy (Papaver somniferum L.) were identified from an expressed sequence tag (EST) database comprised of 20,340 sequences. In total, 2780 SSR-containing sequences were identified. The most frequent microsatellite had an AT/TA motif (37%). Twenty-two opium poppy EST-SSR markers were presently developed and polymorphisms of six markers (psom 2, 4, 12, 13, 17, and 22) were utilized in 135 individuals under narcotic control investigation. An average of three alleles per locus (range: 2–5 alleles) with a mean heterozygosity of 0.167 was detected. Six loci identified 29 unique profiles in 135 individuals. The EST-SSR markers exhibited small degrees of genetic differentiation (fixation index = 0.727, p
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::cee534eff122b7023df3ab3a44642201 https://doi.org/10.1111/j.1556-4029.2011.01810.x Zobrazit plný text záznamu Plný text