Abstract 025: RNAseq And Bioinformatics Identify New Pathways In The Thick Ascending Limb (TAL) Involved In Fructose Induced Salt Sensitive Hypertension

Autor: Andrew Wu, Andrew J Eaton, Paulo Caceres, Puja Shrestha-Maskey, Indra Adrianto, Jessica Granados-Pineda, Mohammed Z Haque, Jose A Garcia Pedraza, Pablo A Ortiz
Rok vydání: 2022
Předmět:
Zdroj: Hypertension. 79
ISSN: 1524-4563
0194-911X
DOI: 10.1161/hyp.79.suppl_1.025
Popis: Elevated fructose consumption is associated with hypertension in humans. In normal Sprague Dawley rats, a high fructose intake (20% fructose drinking water) induced salt sensitive hypertension. We found that fructose directly stimulates the apical Na/K/2Cl cotransporter NKCC2 in the thick ascending limb (TAL), and when consumed for more than 7 days, increases NKCC2 phosphorylation at Thre 96,101. SPAK and OSR1 kinases are known to phosphorylate NKCC2, but it is not known how fructose activates SPAK or OSR1. We hypothesized that fructose intake activates several signaling cascades in TALs that selectively stimulate SPAK. Using an antibody that recognizes activated SPAK and OSR1 (P-Ser 373 SPAK; Ser325 in OSR1), we found that 20% fructose (14 d) enhanced SPAK/OSR1 phosphorylation by 179±32% (p
Databáze: OpenAIRE
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