Cloning of Myrosinase cDNA from Wasabia japonica Matsum

Autor:	Toru Kuboi, Sanshiroh Saitoh, Hideo Etoh, Masakazu Hara
Rok vydání:	2005
Předmět:	Marketing Cloning chemistry.chemical_classification Pungency Myrosinase General Chemical Engineering Biology Industrial and Manufacturing Engineering Petiole (botany) Amino acid Biochemistry chemistry Complementary DNA Specific activity Gene Food Science Biotechnology
Zdroj:	Food Science and Technology Research. 11:412-415
ISSN:	1881-3984 1344-6606
Popis:	Myrosinase (β-thioglucoside glucohydrolase, EC 3.2.3.1) is involved in pungency generation in wasabi (Wasabia japonica Matsum.). We isolated full-length cDNA (WjMY1) encoding myrosinase from the petiole of wasabi. The deduced polypeptide has 545 amino acids with a molecular weight of 62107. WjMY1 mRNA is accumulated in whole organs of the wasabi plant ; the order of expression is petiole≥rhizome>leaf. The order was found to be similar to that of the specific activity of myrosinase. This is the first report de- scribing the isolation of a myrosinase gene from wasabi.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ceb89821389d3f3d273bee99c5780b03 https://doi.org/10.3136/fstr.11.412 Zobrazit plný text záznamu