042 FGF?BP improves wound healing through the induction of more collagen in rats

Autor: Michaela Bittner, Andreas Goppelt, J. Liu, Y. Shi, Jeffrey M. Davidson
Rok vydání: 2004
Předmět:
Zdroj: Wound Repair and Regeneration. 12:A12-A12
ISSN: 1524-475X
1067-1927
Popis: The fibroblast growth factors are important regulators during wound healing. To quench their biological activities, secreted FGFs are tightly bound to heparan sulfate proteoglycans in the extracellular matrix (ECM). One of the approaches for releasing the active FGF from ECM involves the binding to an FGF binding protein (FGF-BP), which prevents FGF degradation and retains its activities. FGF-BP enhances the proliferation of fibroblasts through FGF-1 and -2 and of endothelial cells through FGF-2. To detect the biological function of FGF-BP in wound healing, we constructed an adenoviral vector containing murine FGF-BP cDNA (Ad-FGF-BP). Polyvinyl alcohol (PVA) sponges were implanted subcutaneously in rats. 107–108 PFU of either Ad-FGF-BP or Ad-LacZ (as local control) was injected into the sponges at d3 after implantation. At d4 after injection, Ad-FGF-BP infected sponges displayed much better organization of granulation tissue with the presence of more macroscopic hemorrhage than the local control. At d7 after injection, Ad-FGF-BP infected experimental granulation tissues in sponges showed more collagen deposition. The contents of collagen, protein and DNA in Ad-FGF-BP infected sponges increased 24.0%(p
Databáze: OpenAIRE
Externí odkaz: