Recovery efficiency of a hydrophilic ionic-liquid aqueous biphasic system for the primary purification of cytochrome c from simulated Saccharomyces cerevisiae fermentation broth
| Autor: | Phei Er Kee, Hui Suan Ng, John Chi-Wei Lan, Yu-Yu Lin |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0303 health sciences Chromatography Aqueous solution biology Potassium Cytochrome c Extraction (chemistry) chemistry.chemical_element Bioengineering 01 natural sciences Applied Microbiology and Biotechnology Biochemistry Partition coefficient 03 medical and health sciences chemistry.chemical_compound chemistry Bromide 010608 biotechnology Ionic liquid biology.protein Fermentation 030304 developmental biology |
| Zdroj: | Process Biochemistry. 94:110-115 |
| ISSN: | 1359-5113 |
| Popis: | Ionic liquid-based aqueous biphasic system (ILABS) has emerged as an attractive green approach for the extraction and separation of various biomolecules. The growing market demands of cytochrome c (cyt c) due to its vast uses in medical applications have urged the search for cost-efficient approaches for the production and purification of cyt c. In this study, the feasibility of ILABS to recover cyt c from simulated Saccharomyces cerevisiae cell cultures was investigated by evaluating the effects of phase composition, pH, and additives concentration on the recovery efficiency of cyt c. The ILABS was developed using the hydrophilic ionic liquids, 1-hexyl-3-methylimidazolium bromide ((C6mim)Br) and potassium citrate. The optimal separation conditions for recovery of cyt c from simulated cells cultures were attained with ILABS of pH 9 comprising of 28% (w/w) of (C6mim)Br and 24% (w/w) of potassium citrate and the addition of 0.2% (w/w) NaCl at room temperature (25 °C). Cyt c was recovered in the IL-rich phase with partition coefficient (Kc) of 364.00 ± 1.09, recovery yield (Y) of 99.76% ± 0.03 and selectivity (S) of 223.31 ± 0.56. The results suggest that ILABS can efficiently recover cyt c from microbial fermentation broth with high recovery yields and separation efficiency. |
| Databáze: | OpenAIRE |
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