| Autor: | Anupama B. Athota, Yan Yan, Tricia L. Rozypal, W. S. Chu, J. C. Colclasure, Thomas J. Kelly, R. L. Osborne |
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| Rok vydání: | 1998 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty biology Chemistry Cell General Medicine Matrix metalloproteinase medicine.disease Metastasis Fibronectin Extracellular matrix medicine.anatomical_structure Oncology Invadopodia Cancer cell biology.protein medicine Cancer research skin and connective tissue diseases Batimastat |
| Zdroj: | Clinical & Experimental Metastasis. 16:501-512 |
| ISSN: | 0262-0898 |
| DOI: | 10.1023/a:1006538200886 |
| Popis: | Breast cancer cell lines vary in invasive behavior and one highly invasive cell line (MDA-MB-231)proteolytically degrades extracellular matrix with invadopodia (Thompson et al. 1992, J Cell Physiol, 150,534-44; Chen et al. 1994, Breast Cancer Res Treat, 31, 217-26). Invadopodial proteolysis of extracellularmatrix is thought to be necessary for invasion; however, this has not been demonstrated directly. To obtainsuch evidence, normal (HBL-100) and malignant (MCF-7, MDA-MB-231) breast cells were evaluated forinvadopodial proteolysis of extracellular matrix and invasive behavior. We report that invadopodial prote-olysisof immobilized fibronectin is positively correlated with invasion of cells into type I collagen gels.Moreover, reducing the proteolytic activity of invadopodia with the metalloproteinase inhibitor, batimastat(BB-94), also decreases invasion indicating that breast cancer cell invasion is dependent upon proteolyti-callyactive invadopodia. ©Kluwer Academic Publishers |
| Databáze: | OpenAIRE |
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