The effect of MTHFD2 on the proliferation and migration of colorectal cancer cell lines
| Autor: | Dan Li, Yu Wang, Yanfeng Wang, Yani Chen, Qian Li, Wanjuan Xue, Juan Du, Haiyan Shi, Yun Feng, Jing Zhang, Pengfei Liu, Yi Gao, Jiming Han, Yameng Wei, Huahua Zhang, Jing Yan |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
medicine.diagnostic_test Oncogene Cell growth Cell cycle Biology digestive system diseases Flow cytometry 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Oncology Cell culture Apoptosis 030220 oncology & carcinogenesis Cancer cell medicine Cancer research Pharmacology (medical) MTT assay |
| Zdroj: | OncoTargets and Therapy. 12:6361-6370 |
| ISSN: | 1178-6930 |
| DOI: | 10.2147/ott.s210800 |
| Popis: | Purpose Methylenetetrahydrofolate dehydrogenase 2 (MTHFD2) is a tetramethylfolate dehydrogenase enzyme involved in folate metabolism. The aim of this study is to determine the effect of MTHFD2 on the proliferation and metastasis of colorectal cancer (CRC). Patients and methods MTHFD2 was silenced or overexpressed in CRC cells. qRT-PCR and Western blotting were used to analyze mRNA and protein expression, respectively. The MTT assay and colony forming assay were used to detect cell proliferation and colony formation ability. The cycle and apoptosis changes were detected by flow cytometry. Transwell experiments were used to analyze the migration ability of CRC cells. Results The expression of MTHFD2 in 31 kinds of cancers was analyzed by bioinformatics, and MTHFD2 was found highly expressed in various cancer cells including CRC cells. Silencing the expression of MTHFD2 resulted in inhibition of the proliferation of CRC cells, weakening of the migration ability, blocking of the cell cycle in G0/G1-S phase, and promotion of the apoptosis of CRC cells. On the contrary, overexpression of MTHFD2 in CRC cells resulted in enhancement of the proliferation and migration ability, promotion of cell cycle progression and inhibition of cell apoptosis. Conclusion MTHFD2 is positively related with colorectal cancer and the MTHFD2 gene is a tumor promoting gene in CRC cells. |
| Databáze: | OpenAIRE |
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