Single Nucleotide Polymorphisms of GnRHR Gene and Its Relationship with Reproductive Performance in Egyptian Buffaloes
| Autor: | Ahmed S A Sosa, Mahmoud Abou El-Roos, H. A. A. Eldebaky, Karima Ghoneimy M., M.F. Nawito, Mohamed M.M. Kandiel |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Genetics chemistry.chemical_classification Multiple sequence alignment business.industry GNRHR 0402 animal and dairy science Single-strand conformation polymorphism Single-nucleotide polymorphism 04 agricultural and veterinary sciences 040201 dairy & animal science 03 medical and health sciences 030104 developmental biology chemistry parasitic diseases Genotype Medicine Nucleotide business Gene Blood sampling |
| Zdroj: | Egyptian Journal of Veterinary Sciences. 47:41-50 |
| ISSN: | 2357-089X |
| DOI: | 10.21608/ejvs.2016.1081 |
| Popis: | THIS WORK aimed to search for single nucleotide polymorphisms of gonadotropin releasing hormone (GnRHR) gene in Egyptian buffalo which involved in endocrine control of fertility. An ultrasonographic examinations of anestrum and repeat breeders’ buffaloes wereperformed prior to blood sampling through a transrectal ultrasonography. DNA was extracted from blood and the PCR and single-strand conformation polymorphism (SSCP) techniqueswereadopted to study of GnRHR gene polymorphism. The PCR amplified a fragment with 240-bp in size and the SSCP results showed that there isagenetic polymorphism with three different patterns (AA, BB and CC) in Egyptian buffalo. The CC genotype was associated with smooth inactive ovary,while BB genotype was associated with fertility in buffalo. Multiple sequence alignment of the three patterns sequences revealed that GnRHR had 5 single nucleotide polymorphisms including one nucleotide insertion, one nucleotide deletion and 3 nucleotide substitutions. Insertion was at position 4 in pattern III. The deletion was at position 189 in pattern III, while the 3 nucleotide substitutions were at positions 204 (T/G), 206 (T/A) and 207 (A/T). In conclusion, GnRHR gene could be used as a candidate marker for fertility in Egyptian buffaloes with its mutation is relatedto ovarian inactivity. |
| Databáze: | OpenAIRE |
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