Isolation and partial characterization of endophytic diazotrophs associated with Japanese sugarcane cultivar

Autor:	Shoichiro Akao, Constancio A. Asis, Yasuhiro Arima, Hiroyuki Ohta, Vladimir K. Chebotar, Ken-ichi Tsuchiya, Masatsugu Kubota
Rok vydání:	2000
Předmět:	Herbaspirillum Soil Science Plant Science Biology biology.organism_classification Herbaspirillum seropedicae Rhizobacteria Endophyte Horticulture Saccharum officinarum Most probable number Botany Diazotroph Acetobacter
Zdroj:	Soil Science and Plant Nutrition. 46:759-765
ISSN:	1747-0765 0038-0768
DOI:	10.1080/00380768.2000.10409142
Popis:	Endophytic bacteria were isolated from the juice of a 6 month-old sugarcane cv. NiF-8, which was collected from Miyako, Okinawa and planted in a field lysimeter in Tsukuba, Ibaraki, Japan. The most probable number of N2-fixing endophytes was 4.5 × 105 cells per gram of fresh weight sample and 21 isolates have a positive reaction for ARA in an N2-free semi-solid medium with 10% crystallized sugarcane sugar and 0.5% sugarcane juice. Analyses of some of the biochemical properties of the N2-fixing isolates indicated that 13 isolates were putative strains of Acetobacter diazotrophicus, 4 isolates showed similar characteristics to those of Herbaspirillum seropedicae, and 4 isolates consisted of Herbaspirillum rubribalbicans-like strains. This study confirmed the existence of N2-fixing endophytic bacteria in the Japanese sugarcane cv. NiF-8.
Databáze:	OpenAIRE
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