Fluorescent Nanodiamond – A Novel Nanomaterial forIn VivoApplications

Autor: Nitin Mohan, Bailin Zhang, Chao Sheng Chen, Yi-Chun Wu, Jui Hsia Weng, Cheng-Chun Chang, Hsiao Han Hsieh, Liling Yang, Bon Chu Chung, Chia Yi Fang, Huan-Cheng Chang, Chun Yi Cho
Rok vydání: 2011
Předmět:
Zdroj: MRS Proceedings. 1362
ISSN: 1946-4274
0272-9172
Popis: Fluorescent nanodiamonds (FNDs) with a size in the range of 10 – 100 nm have been produced by ion irradiation and annealing, and isolated by differential centrifugation. Single particle spectroscopic characterization with confocal fluorescence microscopy and fluorescence correlation spectroscopy indicates that they are photostable and useful as an alternative to far-red fluorescent proteins for bioimaging applications. We demonstrate the application by performingin vivoimaging of bare and bioconjugated FND particles (100 nm in diameter) inC. elegansand zebrafishes and exploring the interactions between this novel nanomaterial and the model organisms. Our results indicate that FNDs can be delivered to the embryos of both organisms by microinjection and eventually into the hatched larvae in the next generation. No deleterious effects have been observed for the carbon-based nanoparticlesin vivo. The high fluorescence brightness, excellent photostability, and nontoxic nature of the nanomaterial have allowed long-term imaging and tracking of embryogenesis in the organisms.
Databáze: OpenAIRE
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