Characteristics of a two-site immunoradiometric assay for human skeletal alkaline phosphatase in serum
| Autor: | R Fisher, C Darte, F Singer, William R. Ryan, I Mizrahi, P D Miller, B C Kress, P D Delmas, K Panigrahi, O Reiss |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
musculoskeletal diseases
education.field_of_study Immunoradiometric assay medicine.drug_class Microgram Biochemistry (medical) Clinical Biochemistry Population Liter Biology Monoclonal antibody Isozyme Molecular biology Biochemistry medicine Alkaline phosphatase education Quantitative analysis (chemistry) |
| Zdroj: | Clinical Chemistry. 40:822-828 |
| ISSN: | 1530-8561 0009-9147 |
| DOI: | 10.1093/clinchem/40.5.822 |
| Popis: | This two-site IRMA includes specific monoclonal antibodies for measuring skeletal alkaline phosphatase (B-ALP) in human serum. Assay calibration is based on mass units (micrograms per liter) and was established with purified B-ALP from a human osteosarcoma cell line, SAOS-2. Precision studies demonstrated intra- and interassay CVs of 3-5% and 5-7%, respectively. Relative reactivity studies showed that the assay has a sevenfold preference for detecting B-ALP compared with the liver isoenzyme in serum. The normal reference interval for 478 healthy adults was 5-22 micrograms/L. Method comparison studies showed good correlation between this B-ALP assay (y) and commercially available electrophoretic methods (x) (y = 0.3540x + 20.5, R2 = 0.929) in a pagetic population. Temporal profiles for total ALP, this IRMA B-ALP assay, and B-ALP by electrophoresis in three pagetic patients were parallel. We conclude that this assay demonstrates good analytical performance and would be useful for the clinical assessment of metabolic bone disorders. |
| Databáze: | OpenAIRE |
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