Single reaction interface flow system for chemiluminescent monitoring of mannitol based on its hydroxyl radical scavenger activity

Autor:	Cristina I.C. Silvestre, José L. F. C. Lima, Elias A.G. Zagatto, João L.M. Santos
Rok vydání:	2008
Předmět:	Combinatorial chemistry Scavenger (chemistry) Analytical Chemistry law.invention Luminol chemistry.chemical_compound Reaction interface chemistry Myoglobin law medicine Organic chemistry Hydroxyl radical Sample preparation Mannitol medicine.drug Chemiluminescence
Zdroj:	Talanta. 77:518-521
ISSN:	0039-9140
DOI:	10.1016/j.talanta.2008.03.021
Popis:	A single reaction interface flow analysis (SIFA) system for the monitoring of mannitol in pharmaceutical formulations and human urine is presented. The developed approach takes advantage of the mannitol scavenger aptitude to inhibit the chemiluminescent reaction between luminol and myoglobin in the absence of H2O2. The SIFA system facilitated the fully automation of the developed methodology, allowing the in-line reproducible handling of chemical species with a very short lifetime as is the case of the hydroxyl radical generated in the abovementioned luminol/myoglobin reaction. The proposed methodology allowed the determination of mannitol concentrations between 25 mmol L−1 and 1 mol L−1, with good precision (R.S.D.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ce546d8640d2bbdb6cb8eb57ec7f77e6 https://doi.org/10.1016/j.talanta.2008.03.021 Zobrazit plný text záznamu Full Text from ScienceDirect