Magnetic-Fluorescent Colloidal Nanobeads: Preparation and Exploitation in Cell Separation Experiments
| Autor: | Teresa Pellegrino, Liberato Manna, Massimo Zambianchi, Philomena Piacenza, Giovanna Barbarella, Raffaella Buonsanti, Mariarosaria Musaro, Pantaleo Davide Cozzoli, Roberto Cingolani, Riccardo Di Corato |
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| Rok vydání: | 2009 |
| Předmět: |
Nanostructure
Materials science Polymers and Plastics Magnetic domain Magnetic separation Nanoparticle Bioengineering Nanotechnology Cell sorting equipment and supplies Fluorescence Biomaterials chemistry.chemical_compound chemistry Polymer chemistry Materials Chemistry Magnetic nanoparticles human activities Iron oxide nanoparticles Biotechnology |
| Zdroj: | Macromolecular Bioscience. 9:952-958 |
| ISSN: | 1616-5187 |
| DOI: | 10.1002/mabi.200900154 |
| Popis: | Nanostructures displaying fluorescence and magnetic properties at the same time are potentially useful for achieving simultaneous bio-separation and bio-sensing (e.g., magnetic separation coupled with multiplexing optical detection of different tumour cell populations). Spherical nanobeads that display both fluorescent and magnetic features are reported; they are fabricated by grafting fluorescent oligothiophene molecules to an amphiphilic polymer that is then used to enwrap iron oxide nanoparticles, which acts as the magnetic domain. By tuning experimental conditions, control over the number of magnetic nanoparticles per bead and over the bead diameter (30-400 nm) was achieved. A cell separation efficiency of the level required for cell sorting applications is also reported. |
| Databáze: | OpenAIRE |
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