The inhibition of basal phosphoinositide-dependent phospholipase C activity in Arabidopsis suspension cells by abscisic or salicylic acid acts as a signalling hub accounting for an important overlap in transcriptome remodelling induced by these hormones
| Autor: | Ludivine Soubigou-Taconnat, Ruben Puga-Freitas, Sandrine Balzergue, Alain Zachowski, Anne Repellin, Tetiana Kalachova, Eric Ruelland, V. S. Kravets |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Phospholipase C Phospholipase D organic chemicals fungi food and beverages Plant Science Biology biology.organism_classification 01 natural sciences Transcriptome Wortmannin 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Biochemistry Arabidopsis Phosphatidylinositol phosphorylation Phosphatidylinositol Agronomy and Crop Science Abscisic acid Ecology Evolution Behavior and Systematics 010606 plant biology & botany |
| Zdroj: | Environmental and Experimental Botany. 123:37-49 |
| ISSN: | 0098-8472 |
| DOI: | 10.1016/j.envexpbot.2015.11.003 |
| Popis: | We show that both abscisic acid (ABA) and salicylic acid (SA) inhibit in vivo basal PI-PLC activity in Arabidopsis suspension cells. However, SA also activates in vivo phosphatidylinositol phosphorylation, which ABA does not. The transcriptome response of Arabidopsis suspension cells to a 4 h ABA treatment was compared to that of cells treated with U73122 or wortmannin, inhibitors of phospholipase C (PLC) and of the phosphatidylinositol-4-kinase that provides substrates to PLC, respectively. A clear over representation of genes for which ABA and these inhibitors have similar effects can be observed. Furthermore, 28% of ABA-induced genes were also induced by SA, while 40% of ABA-repressed genes were also repressed by SA. A similarity search in publically accessible transcriptome data confirms that those genes are also similarly regulated in response to ABA and SA in seedlings. We have shown that some of the SA responsive genes responded via an increase in phosphorylated phosphatidylinositol (phosphoinositides), while others did so via the activation of a phospholipase D pathway, and that the SA response of other genes was mimicked by the inhibition of PI-PLC. Interestingly, the pool of genes similarly regulated by SA and ABA is impoverished in genes responsive to SA via phosphoinositides but is enriched in those for which the response to SA is mimicked by the inhibition of PI-PLC. The increase in phosphoinositide appears to be an important process explaining the SA-specific response, while inhibition of a basal PI-PLC might contribute to the common ABA and SA transcriptomic responses. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect