Analysis of multiclass cyanotoxins (microcystins, anabaenopeptins, cylindrospermopsin and anatoxins) in lake waters using on-line SPE liquid chromatography high-resolution Orbitrap mass spectrometry

Autor:	Gabriel Munoz, Audrey Roy-Lachapelle, Dana F. Simon, Sébastien Sauvé, Quoc Tuc Dinh, Emmanuelle Bahl, Sung Vo Duy
Rok vydání:	2019
Předmět:	Detection limit Chromatography General Chemical Engineering Extraction (chemistry) General Engineering High resolution Orbitrap Mass spectrometry Algal bloom Analytical Chemistry law.invention chemistry.chemical_compound chemistry law Environmental science Cylindrospermopsin Surface water
Zdroj:	Analytical Methods. 11:5289-5300
ISSN:	1759-9679 1759-9660
DOI:	10.1039/c9ay01132c
Popis:	Harmful algal blooms (HABs) of cyanobacterial origin have the potential to generate hundreds of secondary metabolites referred to as cyanotoxins. Freshwater resources have been threatened by HABs and an increase of these episodes is of major concern worldwide for risk management and ecosystem impacts. To meet the need to rapidly screen a wide range of cyanotoxins, a multi-toxin method based on on-line solid-phase extraction ultra-high performance liquid chromatography high-resolution mass spectrometry (SPE-UHPLC-HRMS) was developed and validated. This method enabled high-throughput screening of cylindrospermopsin, anatoxin-a, homoanatoxin-a, anabaenopeptins A and B, and twelve microcystins (-RR, [Asp3]-RR, -YR, -HtyR, -LR, [Asp3]-LR, -HilR, -WR, -LA, -LY, -LW and -LF) in recreational lake waters. Extraction and separation were achieved in 8 minutes, with limits of detection between 8 and 53 ng L−1. The method offered suitable precision for environmental samples (generally
Databáze:	OpenAIRE
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