Cholinesterases of aphids—III. Sensitivity of acetylcholinesterases to several inhibitors as a possible phylogenetic character

Autor: E.B. Maizel, S.N. Moralev, E. V. Nikanorova, A.E. Khovanskikh, A.P. Brestkin, K.V. Novozhilov, I.N. Sazonova
Rok vydání: 1989
Předmět:
Zdroj: Insect Biochemistry. 19:15-18
ISSN: 0020-1790
DOI: 10.1016/0020-1790(89)90004-8
Popis: The influence of 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB), three organophosphorous inhibitors (OPI) and physostigmine on the velocity of the acetylthiocholine enzymatic hydrolysis by the homogenates of 35 species of insects of the order Homoptera including 30 species of aphids (Aphidinea) was studied. Acetylcholinesterase (AChE) of the representatives of the suborders psylla (Psyllinea), coccids (Coccinea), whitefly (Aleyrodinea) and leafhoppers (Cicadinea) is not inhibited by DTNB and has lower sensitivity to OPI and higher sensitivity to physostigmine as compared to the AChE of most species of aphids which have been investigated. It was found that only aphid AChE is sensitive to DTNB, this property being less pronounced for AChE of the aphid families Phylloxeridae and Lachnidae than for AChE of the aphid families Callaphididae, Pemphigidae, Chaitophoridae, Anoeciidae, Thelaxidae and Aphididae. AChE of all the aphids under investigation had very similar sensitivity to the OPIs studied and to physostigmine with the exception of AChE of the grape phylloxera Viteus vitifolii Fitch (Phylloxeridae) whose sensitivity to OPI was extremely low. It was concluded that the Lachnidae family is more ancient compared to other contemporary families of viviparous aphids.
Databáze: OpenAIRE