Laccase production in solid-state and submerged fermentation byPleurotus ostreatus

Autor:	Soumendra Kumar Basu, S. K. Mazumder, Mina Mukherjee
Rok vydání:	2009
Předmět:	Laccase Environmental Engineering biology Bioengineering biology.organism_classification Submerged fermentation Microbiology Ferulic acid chemistry.chemical_compound chemistry Solid-state fermentation Yeast extract Degradation (geology) Fermentation Food science Pleurotus ostreatus Biotechnology
Zdroj:	Engineering in Life Sciences. 9:45-52
ISSN:	1618-2863 1618-0240
Popis:	A solid-state fermentation (SSF) system for production of an industrially important enzyme laccase by Pleurotus ostreatus was developed by using potato dextrose yeast extract medium and polyurethane foam as a supporting material. The maximum laccase production in the SSF system was as high as 3�105 U/L. Addition of inducers, such as copper and ferulic acid, further enhanced the laccase production in SSF. Moreover, the time required for the maximum laccase production was reduced to 6 days compared to 10 days reported earlier. The improvement achieved by the SSF system was investigated by comparing it to a submerged fermentation system (SmF), both experimentally and by using a standard theoretical model along with a parameter sensitivity analysis. Laccase production in SSF was found to be twice of that in SmF. One of the main reasons for higher laccase production in SSF compared to SmF was possibly due to the presence of higher proteolytic activity in SmF. Strong proteolytic activity in SmF presumably caused subsequent laccase degradation, which lowered the ultimate laccase production in SmF compared to SSF.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ce0ae5688fc5a68230deecb81eac90a3 https://doi.org/10.1002/elsc.200700039 Zobrazit plný text záznamu