Electrodes Potentiometriques Et Amperometriques a Levures Permeabilisees: Determination Du L-Lactate

Autor:	Gaston Patriarche, Michel Devleeschouwer, Bernard Vincke
Rok vydání:	1985
Předmět:	Reproducibility Chromatography biology Chemistry Biochemistry (medical) Clinical Biochemistry Hansenula anomala Biochemistry Redox Cofactor Amperometry Analytical Chemistry Ionic strength Electrode Electrochemistry biology.protein Biosensor Spectroscopy
Zdroj:	Analytical Letters. 18:593-607
ISSN:	1532-236X 0003-2719
DOI:	10.1080/00032718508066159
Popis:	A biosensor using permeabilized yeasts (Hansenula anomala) is described for the determination of L-lactate. The same electrode can be used either potentiometrically or amperometrically. The linear ranges are respectively of 4.10−5M to 2.10−3M for potentiometry and 8.10−7M to 3.10−3M for amperometry with a reproducibility and a precision of ± 2 to 3%. The parameters involved in the optimization of electrode response, such as pH, temperature, cofactor concentration and ionic strength of the buffers, are discussed in details for both types of measurements. The amperometric technique is suitable for lactate determinations in biological media. In this case, differential measurements are used in order to eliminate interferences of biological redox reactions.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ce03c8482e79c67118bea34f1dd1f091 https://doi.org/10.1080/00032718508066159 Zobrazit plný text záznamu