Isolation, culture and division of cotton callus protoplasts
| Autor: | Edward C. Cocking, J. B. Power, S.S. Bhojwani |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
Ammonium nitrate
fungi food and beverages Soil Science chemistry.chemical_element biochemical phenomena metabolism and nutrition Protoplast Calcium Biology equipment and supplies Isolation (microbiology) Hypocotyl Cell wall chemistry.chemical_compound chemistry Callus Division (horticulture) Botany bacteria Agronomy and Crop Science |
| Zdroj: | Plant Science Letters. 8:85-89 |
| ISSN: | 0304-4211 |
| DOI: | 10.1016/0304-4211(77)90176-6 |
| Popis: | Protoplasts were isolated from hypocotyl callus of Gossypium hirsutum . During culture, protoplasts regenerated a cell wall and subsequently divided with the formation of multicellular colonies. A temperature higher than 25°C and the presence, in the culture medium, of ammonium nitrate and calcium chloride, were required for protoplast survival and division. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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