| Autor: |
Andrew J. Pierce, Dawn M. Stults, Michael W. Killen |
| Rok vydání: |
2014 |
| Předmět: |
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| Zdroj: |
Molecular Toxicology Protocols ISBN: 9781627037389 |
| Popis: |
A newly developed method for quantitatively detecting genomic restructuring in cultured human cell lines as the result of recombination is presented: the "gene cluster instability" (GCI) assay. The assay is physiological in that it detects spontaneous restructuring without the need for exogenous recombination-initiating treatments such as DNA damage. As an assay for genotoxicity, the GCI assay is complementary to well-established sister chromatid exchange (SCE) methods. Analysis of the U-2 OS osteosarcoma cell line is presented as an illustration of the method. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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