Demonstration of a Ribozyme in Epsilon Domain of Hepatitis B Virus RNA

Autor:	Dibyajnan Chakraborty, Sagarmoy Ghosh
Rok vydání:	2020
Předmět:	0301 basic medicine Hepatitis B virus 030102 biochemistry & molecular biology biology Chemistry Pregenomic rna Ribozyme RNA medicine.disease_cause Domain (software engineering) Cell biology 03 medical and health sciences 030104 developmental biology biology.protein medicine
Zdroj:	Methods in Molecular Biology ISBN: 9781071607152
DOI:	10.1007/978-1-0716-0716-9_4
Popis:	The epsilon domain of Hepatitis B virus plays a crucial role in encapsidation of viral pregenomic RNA and its partial NMR structure has been determined. However, we recently described a potassium-dependent ribonucleolytic activity associated with this region, so that a 53 nt long RNA containing the epsilon domain could release itself and cleaved other RNAs. We describe here the experimental methodologies for setting up the reactions and outline a general strategy for initial demonstration of this self-cleaving ribozyme activity.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::cdf327e22c57ced2a6a3dd0f89056382 https://doi.org/10.1007/978-1-0716-0716-9_4 Zobrazit plný text záznamu