Elaboration of novel extraction procedure to reveal bioactive component profile of anthocyanin-rich plants
| Autor: | Peter Forgo, Marietta Korozs, Attila Kiss, Sándor Rapi |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Chromatography
biology DPPH Extraction (chemistry) 04 agricultural and veterinary sciences Ribes biology.organism_classification 040401 food science High-performance liquid chromatography chemistry.chemical_compound 0404 agricultural biotechnology chemistry Anthocyanin Acetone Food science Phosphoric acid Rubus fruticosus Food Science |
| Zdroj: | Czech Journal of Food Sciences. 32:384-390 |
| ISSN: | 1805-9317 1212-1800 |
| Popis: | Kiss A., Rapi S., Korozs M., Forgo P. (2014): Elaboration of novel extraction procedure to reveal bioactive component profile of anthocyanin-rich plants. Czech J. Food Sci., 32: 384–390. The content of anthocyanin derivatives, antioxidant activity, and phenolic content were determined in black elderberry (s ambucus nigra L.), sweet cherry (p runus avium L.), blackberry (Rubus fruticosus L.), black currant (Ribes nigrum L.), and blackthorn ( p runus s pinosa L.). The extraction efficiency was examined of several solvents including hot water, 2% phosphoric acid, ethanol and acetone. A new sequential (cascade) extraction procedure was developed in order to improve the efficiency of the conventional methods. This novel extraction protocol consists of 3 different steps with the prevalence of low pH extraction conditions. When comparing the effectiveness of the conventional and presently improved procedures, it was stated that significantly increased anthocyanin yields had been observed. The highest anthocyanin content, determined with HPLC method, was found in the case of sweet cherry (222.7 mg/kg) on using the three step extraction procedure. The highest antioxidant activity determined with DPPH method was also as signed to the sweet cherry sample (5272 mg/kg). The highest phenolic content was found in blackberry (434 mg/kg). |
| Databáze: | OpenAIRE |
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