Use of allele-specific amplification and analysis of conformation polymorphism for detecting rifampicin resistance of clinical strains ofMycobacterium tuberculosis

Autor:	M. L. Al'tshuler, A G Khomenko, Chernousova Ln, Generozov Ev, Vadim M. Govorun, V. I. Golyshevskaya
Rok vydání:	1999
Předmět:	Genetics Tuberculosis Point mutation General Medicine Rifampicin resistance Biology medicine.disease General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound chemistry Polymorphism (computer science) medicine Polymorphism analysis Allele specific DNA
Zdroj:	Bulletin of Experimental Biology and Medicine. 128:1142-1144
ISSN:	1573-8221 0007-4888
DOI:	10.1007/bf02433423
Popis:	Analysis of 90 clinical strains ofM. tuberculosis demonstrated the possibility of using allele-specific amplification for detecting mutations determining rifampicin resistance. Successive use of two allele-specific primers helped us to detect 85% resistant strains. Other strains were studied using conformation polymorphism analysis of single-stranded DNA fragments. Consecutive use of two assays correctly identified 98% resistant strains.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::cdede5b3d5176cc065f26d2565b7c810 https://doi.org/10.1007/bf02433423 Zobrazit plný text záznamu Full text from SpringerLink