A novel coumarin-based fluorescent sensor for Ca2+ and sequential detection of F− and its live cell imaging
| Autor: | Hao Yang, Kun Yao, Kuoxi Xu, Yongxin Chang, Bai Li |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Detection limit
Fluorescence-lifetime imaging microscopy Aqueous solution Chemistry Metal ions in aqueous solution 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology Photochemistry 01 natural sciences Fluorescence Atomic and Molecular Physics and Optics 0104 chemical sciences Analytical Chemistry Live cell imaging Proton NMR Titration 0210 nano-technology Instrumentation Spectroscopy |
| Zdroj: | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 216:385-394 |
| ISSN: | 1386-1425 |
| DOI: | 10.1016/j.saa.2019.03.035 |
| Popis: | A novel fluorescent sensor CPM for relay detecting Ca2+ and F− based on coumarin has conveniently synthesized and characterized. The sensor CPM showed highly fluorescence enhancement to Ca2+ over other metal ions, and the CPM–Ca2+ complex could selectively recognize F− among other anions. The limits of detection for Ca2+ and F− were 5.81 × 10−7 M and 4.28 × 10−7 M in aqueous solution (DMF/HEPES buffer 1:1 v/v, 10 mM, pH = 7.2), respectively. Their sensing mode had been testified by Job's plots, UV–vis titration, 1H NMR titrations, ESI-mass, fluorescence and DFT calculations. The fluorescence imaging indicated that CPM was cell-permeable and could be used to effectively detect Ca2+ and F− within living cells. |
| Databáze: | OpenAIRE |
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