Assessment of genetic diversity of Burkina Faso sweet grain sorghum using microsatellite markers

Autor: Wend-Pagnangde Marie Serge Felicien Zida, Baloua Nebie, Renan Ernest Traore, J. D. Zongo, Mahamadou Sawadogo, Teyoure Benoit Joseph Batieno, Mahamadi Hamed Ouedraogo, Pauline Bationo-Kando, Zakaria Kiebre, Kiswendsida Romaric Nanema, Nerbéwendé Sawadogo
Rok vydání: 2018
Předmět:
Zdroj: African Journal of Biotechnology. 17:389-395
ISSN: 1684-5315
Popis: Sweet grain sorghum [Sorghum bicolor (L.) Moench] is an under-harvested crop produced mainly for its sweet grains in the pasty stage. Little is known of its genetic diversity remains. This study aims to determine the level and structure of the genetic diversity of sweet grain sorghum from Burkina Faso. Thus, 93 accessions were evaluated using 15 polymorphic microsatellite markers. The analysis revealed 49 alleles in total, 6 rare alleles, an average of 3 alleles per locus, a moderate Nei diversity of 0.474, a low level of heterozygosity (0.031) in the collection and very high Wright's fixation index (Fis) of 0.934. The accessions were organized into three genetic groups: A, B and C. Groups A and B were the farthest, with an Fst and a genetic distance of 0.37 and 0.22, respectively, whereas Groups B and C were the closest, with an Fst (genetic differentiation) of 0.279 and a genetic distance of 0.142. This diversity could be exploited in Burkina Faso sweet grain sorghum breeding programs. Key words: Burkina Faso, neglected culture, sorghum, simple sequence repeats (SSR) markers, genetic variability.
Databáze: OpenAIRE