Abstract 15187: Shear Stress-regulated Mir-27b Controls Pericyte Recruitment in vitro and in vivo and Contributes to Vessel Maturation
| Autor: | Shemsi Demolli, Anuradha Doddaballapur, Konstantin Stark, Reinier A Boon, Ankathrin Eckart, Thomas Korff, Markus Hecker, Yosif Manavski, Tina Lucas, Steffen Massberg, Kavi Devraj, Stefan Liebner, David Kaluza, Stefanie Dimmeler |
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| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Circulation. 132 |
| ISSN: | 1524-4539 0009-7322 |
| Popis: | Vessel maturation involves recruitment of mural cells. Laminar shear stress is a major trigger for vessel maturation. However, the molecular mechanisms by which shear stress affects recruitment of pericytes are unclear. MicroRNAs are small non-coding RNAs, which post-transcriptionally control gene expression. Since shear stress regulates various miRs, we hypothesize that flow-induced miRs inhibit repulsive cues and facilitate mural cell coverage. Laminar shear stress for 72h induces the up-regulation of miR-27b (2.8±0.24-fold vs static, p To determine whether miR-27b controls pericyte recruitment, we tested the effect of endothelial miR-27b on adhesion of pericytes. Overexpression of miR-27b increased the adhesion of pericytes (p Taken together, our data demonstrate that shear stress regulate miR-27b, which targets Ang2 and semaphorins, thereby controlling pericyte adhesion and coverage in vitro and in vivo. |
| Databáze: | OpenAIRE |
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