Thermal stability and folding kinetics analysis of disordered protein, securin
| Autor: | Tzu Hsuan Chen, Chang You Wu, Hsing Yuan Li, Li Yun Tsai, Tsai Mu Cheng, Yao Chen Yang, Hsueh Liang Chu, Li Ping Ho, Chia-Ching Chang, Chia-Seng Chang, Shin Hua Tseng |
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| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | Journal of Thermal Analysis and Calorimetry. 115:2171-2178 |
| ISSN: | 1588-2926 1388-6150 |
| Popis: | Lacking a stable tertiary structure, intrinsically disordered proteins (IDPs) possess particular functions in cell regulation, signaling, and controlling pathways. The study of their unique structural features, thermal stabilities, and folding kinetics is intriguing. In this study, an identified IDP, securin, was used as a model protein. By using a quasi-static five-step (on-path) folding process, the function of securin was restored and analyzed by isothermal titration calorimetry. Fluorescence spectroscopy and particle size analysis indicated that securin possessed a compact hydrophobic core and particle size. The glass transition of securin was characterized using differential scanning microcalorimetry. Furthermore, the folding/unfolding rates (kobs) of securin were undetectable, implying that the folding/unfolding rate is very fast and that the conformation of securin is sensitive to solvent environmental change. Therefore, securin may fold properly under specific physiological conditions. In summary, the thermal glass transition behavior and undetectable kobs of folding/unfolding reactions may be two of the indices of IDP. |
| Databáze: | OpenAIRE |
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