Sequence-responsive unzipping DNA cubes with tunable cellular uptake profiles

Autor:	Karina M. M. Carneiro, Lawrence Panasci, Hanadi F. Sleiman, Joel Neves Briard, Lilian Amrein, Antoine G. Godin, Paul W. Wiseman, Thomas G. W. Edwardson, Graham D. Hamblin, Johans Fakhoury, Katherine E. Bujold
Rok vydání:	2014
Předmět:	media_common.quotation_subject fungi Nucleic acid sequence General Chemistry Cell sorting Biology biology.organism_classification In vitro law.invention HeLa chemistry.chemical_compound Biochemistry chemistry Confocal microscopy law LNCaP Biophysics Internalization DNA media_common
Zdroj:	Chem. Sci.. 5:2449-2455
ISSN:	2041-6539 2041-6520
DOI:	10.1039/c4sc00646a
Popis:	Here, we demonstrate a new approach for the design and assembly of a dynamic DNA cube with an addressable cellular uptake profile. This cube can be selectively unzipped from a 3D to a flat two-dimensional structure in the presence of a specific nucleic acid sequence. Selective opening is demonstrated in vitro using a synthetic RNA marker unique to the LNCaP human prostate cancer cell line. A robust uptake in LNCaP cells, HeLa cells (human cervical cancer) and primary B-lymphocytes isolated from the blood of chronic lymphocytic leukemia (CLL) patients is observed using fluorescence-activated cell sorting (FACS), confocal microscopy and a new cluster analysis algorithm combined with image cross-correlation spectroscopy. The DNA cube was modified with hydrophobic and hydrophilic dendritic chains that were found to coat its exterior. The dynamic unzipping properties of these modified cubes were retained, and assessment of cellular uptake shows that the hydrophobic chains help with the rapid uptake of the constructs while the hydrophilic chains become advantageous for long term internalization.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::cda991a911c66029abce67df7dfb7c8d https://doi.org/10.1039/c4sc00646a Zobrazit plný text záznamu