Evaluation of the effect of narrow band‐ultraviolet B on the expression of tyrosinase, TYRP ‐1, and TYRP ‐2 mRNA in vitiligo skin and their correlations with clinical improvement: A retrospective study
| Autor: | Rowida Ahmed Amer Telep, Noha H. Moftah, Laila A. Rashed, Ahmed Ahmed Touni, Sherif S. Awad |
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| Rok vydání: | 2020 |
| Předmět: |
Messenger RNA
medicine.medical_specialty integumentary system business.industry Tyrosinase Dermatology General Medicine Vitiligo medicine.disease 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine Real-time polymerase chain reaction Depigmentation Endocrinology 030220 oncology & carcinogenesis Internal medicine medicine TYRP1 Tyrosinase-related protein-2 medicine.symptom skin and connective tissue diseases business Gene |
| Zdroj: | Dermatologic Therapy. 34 |
| ISSN: | 1529-8019 1396-0296 |
| DOI: | 10.1111/dth.14649 |
| Popis: | Narrowband-ultraviolet B (NB-UVB) is considered one of the main therapeutic tools in vitiligo, which is able to induce repigmentation and halt depigmentation. However, little remains known about the effect of NB-UVB on TYR gene family, the main pigmentary genes, in vitiligo patients. To assess the effect of NB-UVB on expression of some genes related to the pigmentary problem of vitiligo; tyrosinase (TYR), tyrosinase related protein 1 (TYRP1) and tyrosinase related protein 2 (TYRP2), mRNA levels of those genes were quantitatively evaluated by Real-Time quantitative Polymerase Chain Reaction (RT-qPCR) in skin biopsies obtained from 30 patients with nonsegmental vitiligo and five healthy controls. Vitiligo patients were classified into two groups; group 1, involving 12 untreated vitiligo patients and group 2, including 18 vitiligo patients treated by NB-UVB. The levels of TYR, TYRP-1, and TYRP-2 mRNAs in untreated group were significantly lower than in control subjects (P < .001). In NB-UVB treated group, the three genes were significantly higher than in group 1 (P < .001), however, they were still significantly lower than in the control subjects (P < .001). A significant positive correlation was detected between TYR and TYRP-2 genes in group 2 (P = .03). This study demonstrated that mRNA level of TYR, TYRP-1, and TYRP-2, which decreased in vitiligo, was significantly increased upon treatment with NB-UVB. Accordingly, the mechanism of depigmentation in vitiligo disease and repigmentation by NB-UVB treatment may be related to the changes in the expression of these genes. |
| Databáze: | OpenAIRE |
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