Production of Biosurfactants by Hydrocarbons degrading bacteria isolated from Soummam watershed Sediments of Bejaia in Algeria
| Autor: | Drifa Yalaoui-Guellal, Khodir Madani, Abdelaziz Touati, Fatiha Brahmi, Christophe de Champs, Ibrahim M. Banat |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Environmental Engineering Alcaligenes faecalis Cellulosimicrobium biology Renewable Energy Sustainability and the Environment General Chemical Engineering 030106 microbiology 010501 environmental sciences Biodegradation biology.organism_classification 01 natural sciences Microbiology Pseudomonas stutzeri 03 medical and health sciences Ochrobactrum Environmental Chemistry Yeast extract Food science Waste Management and Disposal Incubation Bacteria 0105 earth and related environmental sciences General Environmental Science Water Science and Technology |
| Zdroj: | Environmental Progress & Sustainable Energy. 37:189-195 |
| ISSN: | 1944-7442 |
| DOI: | 10.1002/ep.12653 |
| Popis: | Hydrocarbons degrading bacteria were isolated from sediments of the Soummam watershed of Bejaia (Algeria). Eleven bacteria strains were isolated using an enrichment technique, method in mineral salt medium, with various hydrocarbons as the sole carbon source. The biodegradation confirmation of various hydrocarbons by these isolates was tested by hole-plate diffusion technique. Out of eleven cultures, nine had shown the growth around the holes. The isolates were screened for biosurfactant producing using oil spreading test and emulsification activity. The value of emulsification activity varied from 55.7 ± 1.1 to 78.5 ± 0.5%. The diameter of clear zone obtained varied between 20.7 ± 1.2 mm and 33.7 ± 1.2 mm, it was hence important compared to the negative control. Five bacterial strains were identified as Alcaligenes faecalis, Ochrobactrum, Cellulosimicrobium, Pseudomonas stutzeri, and Rhodococcus ruber by using physiochemical characterization and MALDI-TOF mass spectrometry tools. The best production of crude biosurfactant by the identified bacterial strains was found in R. ruber which produced 6.7 ± 0.1 g/L of the crude biosurfactant after 168 h incubation in mineral salt medium (MSM) supplemented with 2% of glucose and 0.1 g/L of yeast extract. The biosurfactant produced by all bacterial strains showed a high emulsification index (E24), where P. stutzeri revealed the highest one (92.2 ± 1.1%). © 2017 American Institute of Chemical Engineers Environ Prog, 2017 |
| Databáze: | OpenAIRE |
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