| Autor: |
Maeda Mitsuo, Satoru Shiono, Yoshio Hanazato |
| Rok vydání: |
1990 |
| Předmět: |
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| Zdroj: |
Analytica Chimica Acta. 231:213-220 |
| ISSN: |
0003-2670 |
| DOI: |
10.1016/s0003-2670(00)86419-3 |
| Popis: |
The bienzyme system consisting of glucose oxidase and gluconolactonase was investigated using a conventional diffusion-kinetics model for an enzyme-modified field-effect transistor (FET) to clarify the effect of gluconolactonase coimmobilization in a glucose oxidase membrane on the steady-state response amplitude of a glucose sensor based on a FET. The model includes spontaneous and enzymatic hydrolysis reactions of d -glucono-δ-lactone and it elucidated the following experimental results: a glucose sensor with a membrane (about 1 μm in thickness) coimmobilizing these enzymes showed a sufficient response amplitude, whereas without coimmobilization of gluconolactonase no detectable response was observed up to 3 mM glucose; and the response amplitude depended strongly on the amount of lactonase in the membrane. The model also predicted an optimum enzyme ratio for coimmobilization in a membrane. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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