Combined in vivo recording of neural signals and iontophoretic injection of pathway tracers using a hollow silicon microelectrode
| Autor: | Emese Pálfi, M. Handbauer, Zoltán Fekete, A. Pongrácz, Zs. Bérces, Gergely Márton, István Ulbert, László Négyessy |
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| Rok vydání: | 2016 |
| Předmět: |
Dendritic spine
Analytical chemistry 02 engineering and technology 03 medical and health sciences 0302 clinical medicine In vivo Cortex (anatomy) Materials Chemistry medicine Electrical and Electronic Engineering Axon Instrumentation Biotinylated dextran amine Iontophoresis Chemistry Metals and Alloys 021001 nanoscience & nanotechnology Condensed Matter Physics Surfaces Coatings and Films Electronic Optical and Magnetic Materials Microelectrode Electrophysiology medicine.anatomical_structure 0210 nano-technology 030217 neurology & neurosurgery Biomedical engineering |
| Zdroj: | Sensors and Actuators B: Chemical. 236:815-824 |
| ISSN: | 0925-4005 |
| DOI: | 10.1016/j.snb.2015.12.099 |
| Popis: | This paper presents the results of in vivo local release of a neuronal tracer, biotinylated dextran amine (BDA) in the rat somatosensory cortex using monolithically integrated microfluidic channel of a silicon neural microelectrode. The tracer injection is controlled by iontophoresis using Pt electrodes in the vicinity of the outlet of the microfluidic channel. Using 3–5 μA, 5–7 s on/off cycle and 15–20 min total injection time the localized injection resulted in clear anterograde and retrograde BDA labeling both within the cortex and in subcortical structures. Anterograde and retrograde labeling revealed the fine details of neuronal processes including dendritic spines and axon terminal-like endings. Injection sites appeared clear lacking any strong diffuse background labeling. Electrophysiological recording performed with the same microdevice immediately after the iontophoresis indicated normal cortical functioning. The results prove that the combination of in vivo multichannel neural recording and controlled tracer injection using a single implanted microdevice is feasible, and therefore it can be a powerful tool for studying the connectome of the brain. |
| Databáze: | OpenAIRE |
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