Triplex real-time PCR detection of three quarantine Phytophthora pathogens infecting Malus Miller
| Autor: | Lin-Hui Zhu, Dajin Lv, Guan-Rong Li, Fang Liao, Jia-Feng Luo, Zhang Ying, Baohong Cao |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Malus biology Plant Science Horticulture biology.organism_classification 01 natural sciences Microbiology law.invention 03 medical and health sciences genomic DNA 030104 developmental biology Phytophthora cambivora law Phytophthora syringae Phytophthora Agronomy and Crop Science Gene Polymerase chain reaction 010606 plant biology & botany Phytophthora hibernalis |
| Zdroj: | Journal of Plant Diseases and Protection. 125:325-330 |
| ISSN: | 1861-3837 1861-3829 |
| DOI: | 10.1007/s41348-017-0144-2 |
| Popis: | In order to develop a simultaneous and specific molecular detection of the three quarantine Phytophthora pathogens, Phytophthora hibernalis, Phytophthora cambivora and Phytophthora syringae that infect Malus Miller, three pairs of real-time PCR primers (PH-F/PH-R, PC-F/PC-R and PS-F/PS-R) and three probes (PH-Pr, PC-Pr and PS-Pr) labeled with HEX, FAM and ROX, respectively, were designed for P. hibernalis, P. cambivora and P. syringae by alignment analyses of enolase (Enol), ras-like protein Ypt1 and HSP90 gene sequences with other Phytophthora spp. Black Hole Quencher 1 (BHQ1) was used for P. hibernalis and P. cambivora and BHQ2 for P. syringae. Through the optimization of the reaction conditions, a triplex real-time PCR simultaneous detection for the three Phytophthora species infecting Malus Miller was developed. It could achieve simultaneous and specific detection with a sensitivity of 2 × 10−4, 2 × 10−4 and 2 × 10−2 ng/μL genomic DNA, respectively, for P. hibernalis, P. cambivora and P. syringae. |
| Databáze: | OpenAIRE |
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