Autor: |
P.A. Castaldi, H. Michel, I. Smith, J.M. Connellan |
Rok vydání: |
1977 |
Předmět: |
|
Zdroj: |
VIth International Congress on Thrombosis and Haemostasis. |
ISSN: |
2567-689X |
DOI: |
10.1055/s-0039-1680644 |
Popis: |
Contact factors are known to be associated with platelets. There is evidence to suggest that F XI may mediate an enhancement of coagulant activity in response to collagen stimulation of platelets. The aim of this study was to further explore this relationship using fresh platelets and extracts prepared from normals and a F XI deficient subject. Antisera against plasma F XII and F XI were used in tests of collagen induced coagulant activity and the latter antibody in immunoprecipitation studies with platelet extracts. Comparisons were made with antisera to isolated platelet membranes.Collagen induced coagulant activity was inhibited by anti F XI and to a slight degree by anti F XII. F XI-like coagulant activity could be detected in extracts of washed platelets and this activity adsorbed to heparin-sepharose as does plasma F XI. Similar activity could be detected in platelet extracts from the F XI deficient patient. Normal platelets contained an antigen that precipitated with anti F XI.F XI activity and antigen are closely associated with platelets. This may provide a mechanism for enhancement of coagulation. |
Databáze: |
OpenAIRE |
Externí odkaz: |
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