| Popis: |
Publisher Summary This chapter discusses the regeneration of functional hemoglobin from partially oxidized hemoglobin in the presence of molecular hydrogen and a multicomponent redox catalyst. Molecular hydrogen offers a significant advantage over the reducing agents because it is mild and its oxidation product, H + , can be buffered easily. Polymeric redox catalysts offer a promising approach to the use of molecular hydrogen for methemoglobin reduction. Multicomponent redox catalysts are synthesized in which atoms of metallic platinum are imbedded in an electroactive polymer synthesized from a monomeric bipyridyl derivative. For convenience, the polymeric catalyst is coated onto an inert material with high surface area. With such a catalyst, molecular hydrogen regenerates functional hemoglobin from partially oxidized hemoglobin. Reversible O 2 binding is restored for regenerated ferrous hemoglobin, with only slight reductions in cooperativity and hemoglobin stability. The advantages of the reduction system include the following considerations: (1) the heterogeneous catalyst avoids the problem of protein adsorption onto bare platinum, (2) catalyst and reducing agent are easily removed from the protein by simple filtration, and (3) the by-product, H + is buffered easily. |
