| Autor: |
Salzmann J-L, Noguiez-Hellin P, David Klatzmann, Merten O W, Dante J, Laune S |
| Rok vydání: |
1997 |
| Předmět: |
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| Zdroj: |
Animal Cell Technology ISBN: 9789401062732 |
| DOI: |
10.1007/978-94-011-5404-8_54 |
| Popis: |
The use of animal derived products like trypsine or serum presents a risk due to the potential transmission of adventitious agents. Their replacement by non animal derived or pharmaceutical grade substances is the best choice for avoiding this risk. Trypsin, a widely used substance for the detachment of anchorage dependent cells, can be replaced by heparin in most of the cases. At a concentration of 100IU/ml in the presence of EDTA at 0.02% in PBS. 75% (9/12) of all cell lines tested could be detached from polystyrene based growth supports (T-flasks, roller bottles, the Costar CellCube) without the need of optimizing the detachment conditions. These so detached cells could easily be subcultivated whereby a washing step for eliminating the detachment solution was preferable. The comparison of the growth rates of these subcultures revealed only insignificant differences for cells detached by trypsin/ EDTA, heparin/EDTA or EDTA alone (e.g. for BHK21 C13, recombinant CHO and CRIP M11 cells grown on polystyrene surfaces). |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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