Proliferation and Differentiation of Ependymal Cells after Transection of the Carp Spinal Cord
| Autor: | Toshihiko Miyake, Hajime Yamada, Tadahisa Kitamura |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Ependymal Cell
biology Endoplasmic reticulum Anatomy Golgi apparatus biology.organism_classification Spinal cord Proliferating cell nuclear antigen Lesion symbols.namesake medicine.anatomical_structure medicine symbols biology.protein Immunohistochemistry Animal Science and Zoology medicine.symptom Carp |
| Zdroj: | Zoological Science. 14:331-338 |
| ISSN: | 0289-0003 |
| DOI: | 10.2108/zsj.14.331 |
| Popis: | Proliferation and differentiation of ependymal cells in the injured carp spinal cord were studied by immunohistochemistry using proliferating cell nuclear antigen (PCNA) and 3H-thymidine (3H-TdR) autoradiography. A surge of proliferation of ependymal cells occurred in the lesion with a peak around 6th day after surgical operation (complete transection) of the spinal cord. The proportion of PCNA-positive ependymal cells at 6th post-operative day (6 POD) was over 14 times that in the normal state. Electron microscopic autoradiography revealed that most of the ependymal cells incorporating 3H-TdR at 5 POD, especially those located in the caudal side of the transection site, contained numerous free ribosomes in their apical portion, but the other organellae, such as rough endoplasmic reticulum and Golgi apparatus, were poorly developed in this portion. In three weeks thereafter, the ependymal cell layer was reconstructed through the lesion, and the apical part of the 3H-TdR-labeled ependymal cells be... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|